Reparative dentin formation using stem cell therapy versus calcium hydroxide in direct pulp capping: an animal study

Abstract Direct pulp capping process is a therapeutic method aimed at maintenance of pulp vitality and health by using a biocompatible material placed directly over the exposed pulp. The aim of this study was to evaluate and compare the effect of direct pulp capping procedures by dental pulp stem cells (DPSCs) or calcium hydroxide on dentin tissue formation. Three mongrel dogs were used as experimental model. Two access cavities were prepared in the right and left mandibular fourth premolars in all dogs to expose and extirpate the pulp tissues which were processed in the lab to obtain a single-cell suspensions. The isolated cells were cultures in odontogenic medium for subsequent differentiation. The maxillary teeth (3 incisors and one canine) of the corresponding dog number were subjected to class V cavities to expose their pulps which were assigned into 2 groups of 12 teeth each ( group I - pulp capping with calcium hydroxide) and (group II - pulp capping with dental stem cells DPSCs). The operated teeth were collected after 3 months and processed for histological and electron microscopic examinations. Specimens were subjected to elemental analysis of calcium and phosphorus. EDX elemental analysis revealed significant differences in the calcium and phosphorous wt, % in the reparative dentin of calcium hydroxide treated group which confirmed histologically. Direct pulp capping by DPSCs has shown promising generative potential for regaining normal dentin.

Resumo O processo de capeamento pulpar direto é um método terapêutico que visa a manutenção da vitalidade e saúde da polpa, usando um material biocompatível colocado diretamente sobre a polpa exposta. O objetivo deste estudo foi avaliar e comparar o efeito do capeamento pulpar direto usando células-tronco da polpa dentária (CTPDs) ou hidróxido de cálcio sobre o tecido da dentina formação. Três cães sem raça definida foram utilizados como modelo experimental. Duas cavidades de acesso foram preparados nos quartos pré-molares inferiores direito e esquerdo em todos os cães para expor e extirpar os tecidos pulpares que foram processados ​​em laboratório para obter suspensões de uma célula única. As células isoladas foram cultivadas em meio odontogênico para posterior diferenciação. Os dentes superiores (3 incisivos e um canino) do respectivo cão foram submetidos ao preparo de cavidades classe V para expor suas polpas, as quais foram atribuídas a 2 grupos de 12 dentes cada (grupo I - capeamento pulpar com hidróxido de cálcio) e (grupo II - capeamento pulpar com CTPDs). Os dentes operados foram extraídos após 3 meses e processado para exames histológicos e por microscopia eletrônica de varredura. Os espécimes foram submetidos a análises elementares de cálcio e fósforo. Análise elementar por EDX revelaram diferenças significativas no peso de cálcio e fósforo, % na massa reparadora dentina do grupo tratado com hidróxido de cálcio, o que foi confirmado histologicamente. O capeamento pulpar com CTPDs mostrou potencial promissor para recuperar a dentina normal.

Mastoid process syndrome: an obscure cause for pain in the neck

This article gave a report on 12 patients with a pain syndrome arising from the region of the tip of mastoid process and sternomastoid muscle. It is often missed and yet is readily treatable it is not uncommon cause for pain in the neck treatment suggested was oral and topical non-steroidal anti-inflammatory drugs which control the symptoms. Better recognition of this pain syndrome will save patients from undergoing unnecessary investigation and inappropriate treatment